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Catalog# Product Description |
ICP0615 The biotin antibody is developed using biotin-KLH conjugates and purified with biotin-agarose affinity chromatography. The purified biotin antibody was chemically linked to beaded agarose through a stable covalent linkage. The immobilized biotin antibody on beaded agarose could be utilized for reversible immunoprecipitation and affinity purification of both biotinylated antibodies and biotinylated proteins. This product has been applied to the MS-based proteomics experiments as per paper (Udeshi et al in Nature Methods Vol.14 pages: 1167-1170 (2017)). |
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Formulation
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1 mL beads in 2 mL slurry with glycerol
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Antibody Immobilized
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10 mg/mL agarose
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Applications
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IP; Immuno-affinity chromatography; |
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Scientific Description
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Biotin is a vitamin, which binds strongly and irreversibly to avidin. Biotin is commonly used for labelling reagents. Anti-biotin antibody binds to biotin strongly but reversibly, the binding of the biotinylated species could be competitively eluted with free biotin.
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Storage & Stability
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