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Catalog# Product Description |
ICP1788 This product is cross-linked Aflatoxin M1 to Horse radish peroxidase (HRP). It is useful as an antigen-labelled peroxidase paired with the anti-Aflatoxin antibody in a direct ELISA.
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Structure of the AFM1-HRP Conjugates 
Direct competitive inhibition ELISA using immobilized anti-AFM1 (ICP1798, 0.2 ug/uL) and AFM1-HRP (ICP1788, 0.1 μg/mL), 50% inhibition occurred at 0.5-1 ng/mL of free AFM1 in a non-optimized conditions
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| Formulation |
Lyophilized powder.
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Conjugation |
Aflatoxin M1 methylcarboxyloxime linked to HRP via amine acylation. AFB1/HRP molar ratio:1:1
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| ELISA Titer |
OD50 ELISA titer for AFM1-HRP is approximately 0.05-0.1 ug/mL in plate immobilized with 0.25 ug /mL of rabbit anti-AFM1 (ICP1798). OD signal was 50% inhibited by 1 ng/mL of free AFM1
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Applications |
Direct ELISA : suggested use concentration: 0.1-0.25 μg/mL
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Storage & Stability |
Product is stable for at least 30 days at room temperatures. For extended storage, store at –20oC. Expire date is six months from the date of shipping if stored properly.
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