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Catalog# Product Description |
ICP9807 Affinity purified rabbit polyclonal phospho-threonine antibody (anti-pT) was developed using a phospho-threonine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-threonine specific antibody was affinity purified with phospho-threonine immobilized on agarose. |
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Lane1: Untreated kinase
Lane 2: Kinase treated with phosphatase PP1G
Lane 3: MW marker time course
 Western blot analysis of mouse extract spleen with ICP9807
A: Extract of spleen from mouse treated with sodium vanadate
B: Extract of spleen from control mouse
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Species
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Rabbit
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Formulation
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250 μg/mL in PBS, 50% glycerol, pH 7, 0.01% NaN3 |
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Immunogen
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Phospho-threonine KLH conjugates and phosvitin mixture
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Purification
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The antibody was affinity purified with phospho-threonine on agarose followed by affinity absorption with threonine on agarose.
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Specificity
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The antibody recognizes proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phospho-tyrosine.
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Species Tested
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Human and mouse
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Applications
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ELISA; WB; IP
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Scientific Description
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Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phospho-threonine which enters various biosynthetic pathways in the body.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
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Product Specific References |
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